Thermo Fisher Scientific proBDNF Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200

-

Immunohistochemistry (Frozen) (IHC (F))

1:100

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Guinea pig / IgG

Class

Polyclonal

Type

Antibody

Immunogen

(C)DEDQKVRPNEENNKDAD, corresponding to amino acid residues 72-88 of human BDNF (precursor), Pro-domain of the BDNF protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:proBDNF,uniProtId:P23560-1,ncbiNodeId:9606,antigenRange:72-88,antigenLength:247,antigenImageFileName:ANT-006-GP200UL_proBDNF_P23560-1_Guinea_pig.svg,antigenImageFileNamePDP:ANT-006-GP200UL_proBDNF_P23560-1_Guinea_pig_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 X g 5 min).

Target Information

BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimers and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.