Thermo Fisher Scientific DNMT3A Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Positive Control: 293T, HeLa, HepG2, Neuro2A, PC-12, Rat2, CL1-0
• Predicted Reactivity: Bovine (92%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Methylation of DNA at cytosine residues plays an important role in the regulation of gene expression, genomic imprinting, and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with the development of cancer.
Three families of mammalian DNA methyltransferase genes have been identified: Dnmt1, Dnmt2, and Dnmt3.

• Dnmt1: Constitutively expressed in proliferating cells; inactivation causes global demethylation and embryonic lethality.
• Dnmt2: Expressed at low levels in adult tissues; inactivation does not affect DNA methylation.
• Dnmt3 (Dnmt3a, Dnmt3b): Strongly expressed in embryonic stem cells; expression decreases during differentiation and is low in adult tissues.
  Mutations in the Dnmt3b gene are associated with ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.