Thermo Fisher Scientific GeneArt CRISPR Nuclease Vector with CD4 Enrichment Kit (with competent cells)

Invitrogen™ GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit (with competent cells)

The GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit is a vector system for expression of the functional components needed for CRISPR/Cas9 genome editing in mammalian cells with a CD4 reporter.
The CD4 reporter enables bead-based enrichment, allowing magnetic bead-based sorting/enrichment of Cas9 & CRISPR expressing cells using Dynabeads™ CD4 magnetic beads.
Transfection efficiency can also be tracked using anti-CD4 fluorescent antibodies.

The linearized GeneArt™ CRISPR nuclease vectors provide a rapid and efficient way to clone double-stranded oligonucleotides encoding a crRNA representing a desired target into an expression cassette that allows sequence-specific targeting of the Cas9 nuclease.
This version of the kit includes OneShot™ TOP10 Competent Cells.
A version without competent cells is also available (Cat. No. A21175).

주요 특징

• Easy-to-design genome engineering system
• Affordable, ready-to-use cloning vectors
• Enrichment for hard-to-transfect or difficult-to-modify cell lines
• Competent cells included for added convenience and highest cloning efficiencies

All-in-One Vector System for CRISPR-based Genome Editing

The GeneArt™ CRISPR Nuclease Vector kit offers a simple, ready-to-use, all-in-one expression vector system consisting of both a Cas9 nuclease expression cassette and a guide RNA (gRNA) cloning cassette for rapid and efficient cloning of DNA that encodes target-specific CRISPR RNA (crRNA).
This system allows you to edit and engineer a genomic locus of choice in a sequence-specific manner from a single plasmid.
After relevant targets have been identified with fast and easy-to-use GeneArt™ CRISPR vectors, the biologically relevant mutations can be precisely created with GeneArt™ Precision TALs, with high specificity and low off-target effects.

CRISPR gRNA 디자인 지원

Thermo Fisher의 CRISPR Search & Design Tool을 통해 인간 및 생쥐 유전자 데이터베이스(>600,000 predesigned CRISPR gRNAs) 검색 또는 사용자 서열 기반 de novo gRNA 디자인 가능.
CRISPR 서열은 유전자 knockout에 최적화되어 있으며, 각 유전자의 처음 세 개 전사 엑손을 타겟팅합니다.
검색 결과에는 PAM 사이트, 엑손 맵, gRNA 결합 위치, 잠재적 오프타겟 정보가 포함됩니다.
Start designing today >

Resources

• Demonstrated protocol: Engineering stem cells with CRISPR-Cas9
• Demonstrated protocol: CRISPR-Cas9 microinjection in mice and zebrafish embryos

제품 사양