Thermo Fisher Scientific Phospho-MKP1/MKP2 (Ser296, Ser318) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human DUSP1(Accession P28562), corresponding to amino acid residues around phosphorylated Ser296. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MKP1/MKP2,uniProtId:P28562-1,ncbiNodeId:9606,antigenRange:296,antigenLength:367,antigenImageFileName:PA5-104854_MKP1MKP2_P28562-1_Rabbit.svg,antigenImageFileNamePDP:PA5-104854_MKP1MKP2_P28562-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:MKP1/MKP2,uniProtId:Q13115-1,ncbiNodeId:9606,antigenRange:296|318,antigenLength:394,antigenImageFileName:PA5-104854_MKP1MKP2_Q13115-1_Rabbit.svg,antigenImageFileNamePDP:PA5-104854_MKP1MKP2_Q13115-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2816327

Product Specific Information

Antibody detects endogenous levels of MKP1/2 only when phosphorylated at Serine 296/318.

Target Information

There are ten mitogen-activated protein kinase (MAPK) phosphatases (MKPs) that act as negative regulators of MAPK activity in mammalian cells. MKP2 (DUSP4) is a dual threonine/tyrosine phosphatase that specifically dephosphorylates and inactivates MAPKs (ERK, JNK, p38). It contains one Rhodanese domain and one tyrosine-protein phosphatase domain. MKP2 is expressed in a variety of tissues, and is localized in the nucleus. Transcription factor E2F-1 acts as a transcriptional regulator of MKP2. E2F-1/MKP2 pathway mediates apoptosis under oxidative stress and that MKP2 suppresses tumor formation. MKP2 is a novel transcription target of p53 in mediating apoptosis by 10-bp palindrome motif (CTGGCGCCAG) in the MKP2 promoter.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.