Thermo Fisher Scientific Phospho-PI3K p85 alpha (Tyr607) Polyclonal Antibody
PI3K p85 alpha의 Tyr607 인산화 형태를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF 등 다양한 응용에 적합. Human, Mouse, Rat, Pig 반응성. 고순도 정제 및 안정적인 PBS/glycerol buffer 보존.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 8 publications |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 | - |
| Immunohistochemistry (Frozen) (IHC-F) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Pig, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human PIK3R1 (Accession P27986), corresponding to amino acid residues around phosphorylated Tyr607. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816326 |
Product Specific Information
This antibody detects endogenous levels of PI3K p85 alpha only when phosphorylated at Tyrosine 607.
Target Information
Phosphatidylinositol 3-kinase (PIK3) is a signaling protein that phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. Signaling is initiated when upstream tyrosine kinase receptors or G protein-coupled receptors are bound by their ligands. It binds to activated (phosphorylated) protein-Tyr kinases through its SH2 domain and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.
Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. During insulin stimulation, it also binds to IRS-1. Mutations in the gene can result in Agammaglobulinemia 7, SHORT syndrome, or Immunodeficiency 36.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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