
Thermo Fisher Scientific ENT1 Polyclonal Antibody
Thermo Fisher Scientific의 ENT1 Polyclonal Antibody는 인간 및 마우스 시료에 반응하는 Rabbit IgG 항체로, Western blot, IHC, ICC/IF, Flow cytometry에 적합합니다. 고순도 항원 친화 크로마토그래피로 정제되었으며, 세포 내 뉴클레오사이드 수송 연구에 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within Human ENT1 (aa 19–68) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2901100 |
Product Specific Information
Positive controls include:
- Human plasma lysates
- JAR
- Human breast carcinoma tissue
- Human kidney tissue
- Mouse small intestine tissue
- EA.hy926
Target Information
The ENT1 gene belongs to the equilibrative nucleoside transporter family. It encodes a transmembrane glycoprotein localized to plasma and mitochondrial membranes, mediating cellular uptake of nucleosides. This equilibrative transporter is sensitive to inhibition by nitrobenzylthioinosine. Nucleoside transporters are essential for nucleotide synthesis in cells lacking de novo synthesis pathways and are crucial for uptake of cytotoxic nucleosides used in cancer and antiviral therapies. Multiple alternatively spliced variants encoding the same protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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