Thermo Fisher Scientific UNG Monoclonal Antibody (OTI1B6), TrueMAB

Applications and Tested Dilutions

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

The human UNG gene encodes both nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-DNA glycosylase. These isoforms arise from alternative splicing and use of two differentially regulated promoters (PA and PB).
UNG1 and UNG2 cDNAs are similar in size (2061 and 2058 bp), making them indistinguishable on gel electrophoresis. Nuclear UNG2 differs from mitochondrial UNG1 by 44 amino acids at the N-terminus, which are not required for catalytic activity.
UNG proteins play a crucial role in repairing mutagenic U:G mispairs caused by cytosine deamination. UNG2 removes misincorporated dUMP residues. Expression levels of UNG1 and UNG2 vary by cell and tissue type and are regulated by the cell cycle.
In general, UNG expression and activity are higher in proliferating cells compared to nonproliferating ones. UNG1 is a 304 amino acid protein, while UNG2 consists of 313 amino acids.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.