Thermo Fisher Scientific GAPDH Monoclonal Antibody (1G5)
GAPDH 단백질을 인식하는 Thermo Fisher Scientific의 Mouse Monoclonal Antibody (Clone 1G5). Western blot, ELISA, IP 등 다양한 응용에 적합. 인간 단백질에 반응하며, 액상 형태로 제공되고 보존제가 없음. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| ELISA | 1 ng/mL |
| Immunoprecipitation (IP) | 1–5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1G5 |
| Immunogen | GAPDH (NP_002037, 226–335 a.a.) partial recombinant protein with GST tag. MW of GST tag alone is 26 kDa. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
GKLTGMAFRV PTANVSVVDL TCRLEKPAKY DDIKKVVKQA SEGPLKGILG YTEHQVVSSD FNSDTHSSTF DAGAGIALND HFVKLISWYD NEFGYSNRVV DLMAHMASKE
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme involved in glycolysis. It exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphate in the presence of NADPH.
Beyond glycolysis, GAPDH is ubiquitously expressed and participates in DNA replication, repair, nuclear RNA export, membrane fusion, and microtubule bundling. It plays roles in apoptosis, neurodegenerative diseases such as Alzheimer’s and Huntington’s, and interacts with proteins like amyloid precursor protein and Huntingtin.
Due to its high expression in most tissues, GAPDH serves as a reliable protein loading control in Western blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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