Thermo Fisher Scientific UGGT1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1456-1555 of human UGGT1 (NP_0645051) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:UGGT1,uniProtId:Q9NYU2-1,ncbiNodeId:9606,antigenRange:1456-1555,antigenLength:1555,antigenImageFileName:PA5-92309_UGGT1_Q9NYU2-1_Rabbit.svg,antigenImageFileNamePDP:PA5-92309_UGGT1_Q9NYU2-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.96 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2806435

Product Specific Information

Immunogen sequence: PNNMIHQVPI KSLPQEWLWC ETWCDDASKK RAKTIDLCNN PMTKEPKLEA AVRIVPEWQD YDQEIKQLQI RFQKEKETGA LYKEKTKEPS REGPQKREEL; Positive Samples: SH-SY5Y, 293T, HepG2, HL-60, HT-1080, Mouse liver, Mouse kidney, Rat liver, Rat brain; Cellular Location: Endoplasmic reticulum lumen, Endoplasmic reticulum-Golgi intermediate compartment

Target Information

UDP-glucose:glycoprotein glucosyltransferase (UGGT) is a 170 kDa lumenal endoplasmic reticulum (ER) resident protein that catalyzes monoglucosylation of high mannose oligosaccharides. UGGT catalyzes the transfer of glucose from UDP-glucose to Man7-9GlcNAc2-moiety of unfolded glycoproteins in a Ca2+ dependent reaction. UGGT binds unfolded glycoprotein via two elements on its substrate: a N-acetylglucosamine unit on the oligosaccharide and hydrophobic amino acids that are exposed in the denatured conformation. It is postulated that UGGT serves as a sensor that is able to distinguish between folded and denatured protein in the ER.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.