Thermo Fisher Scientific UGGT1 Polyclonal Antibody
UGGT1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, Human, Mouse, Rat 시료에 반응합니다. Western Blot과 ELISA에 적합하며, ER 내 단백질 접힘 감지 연구에 활용됩니다. 고순도 친화 크로마토그래피 정제, 액상 형태로 제공됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
ELISA
- Tested Dilution: 1 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1456–1555 of human UGGT1 (NP_0645051) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.96 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2806435 |
Product Specific Information
- Immunogen sequence:
PNNMIHQVPI KSLPQEWLWC ETWCDDASKK RAKTIDLCNN PMTKEPKLEA AVRIVPEWQD YDQEIKQLQI RFQKEKETGA LYKEKTKEPS REGPQKREEL - Positive Samples: SH-SY5Y, 293T, HepG2, HL-60, HT-1080, Mouse liver, Mouse kidney, Rat liver, Rat brain
- Cellular Location: Endoplasmic reticulum lumen, Endoplasmic reticulum-Golgi intermediate compartment
Target Information
UDP-glucose:glycoprotein glucosyltransferase (UGGT) is a 170 kDa lumenal endoplasmic reticulum (ER) resident protein that catalyzes monoglucosylation of high mannose oligosaccharides. UGGT catalyzes the transfer of glucose from UDP-glucose to Man7-9GlcNAc2 moiety of unfolded glycoproteins in a Ca²⁺ dependent reaction. It binds unfolded glycoproteins via two substrate elements: an N-acetylglucosamine unit on the oligosaccharide and hydrophobic amino acids exposed in the denatured conformation. UGGT acts as a sensor distinguishing between folded and denatured proteins in the ER.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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