Thermo Fisher Scientific Human IL-3 (Carrier-Free) Recombinant Protein, eBioscience

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-Dependent

-

Control (Ctrl)

Assay-Dependent

-

Product Specifications

Expression System

E. coli

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Storage conditions

-20°C

Shipping conditions

Dry ice

Product Specific Information

Description: Interleukin 3 (IL-3), also called Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines. Human and mouse IL-3 share only 29% sequence identity at the amino acid level. The bioactivity of IL-3 is highly species specific. Human IL-3 has no activity on mouse cells.

Applications Reported: Human IL-3 Recombinant Protein Carrier-Free is biologically active.

Applications Tested: The bioactivity of this protein was determined by its ability to induce proliferation of human TF-1 cells. The ED50 for this is less than or equal to 0.1 ng/mL, which corresponds to a specific activity of greater than or equal to 1x10^7 Units/mg.

Source: E. coli-expressed amino acids Ala20-Phe152 (Accession #NM_000588).

Bioactivity: The bioactivity of this protein was determined by its ability to induce proliferation of human TF-1 cells. The ED50 for this is less than or equal to 0.1 ng/mL, which corresponds to a specific activity of greater than or equal to 1x10e7 Units/mg.

Endotoxin: Less than 0.1 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE and HPLC.

Molecular Weight: 15 kDa.

Storage and handling: Use in a sterile environment. Store lyophilized protein at less than or equal to -20°C. Reconstituted protein solution can be stored with carrier protein (e.g., 0.1% BSA) at less than or equal to -20°C.

Target Information

Interleukin 3 (IL-3), also called Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines. Human and mouse IL-3 share only 29% sequence identity at the amino acid level. The bioactivity of IL-3 is highly species specific. For example, human IL-3 has no activity on mouse cells. IL-3 is a potent growth promoting cytokine. IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types, and is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.