Thermo Scientific™

GeneBLAzer™ FXR DA Assay Kit

GeneBLAzer™ FXR DA (Division Arrested) cells and FXR-UAS-bla HEK 293T 293 cells contain the ligand-binding domain (LBD) of the human자세히 알아보기

GeneBLAzer™ FXR DA (Division Arrested) cells and FXR-UAS-bla HEK 293T 293 cells contain the ligand-binding domain (LBD) of the human farnesoid X receptor (FXR) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™ UAS-bla HEK 293T cell line. GeneBLAzer™ UASbla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-FXR (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both FXR DA cells and FXR-UAS-bla HEK 293T cells are functionally validated for Z` and EC50 concentrations of chenodeoxycholic acid (CDCA). In addition, FXR-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

사양

대체 작용제GW 0742, Retinoic Acid, GW 7647, Bezafibrate

길항제MK886

어세이 입력Cell-based beta lactamase reporter gene

셀 상태Division-Arrested Cells

검출 방법Fluorescence

유전자 기호FXR

일차 작용제L-165, 041

제품라인GeneBLAzer™

수량1 plate

리포터 유전자BLA (Beta-Lactamase)

타겟 입력FXR, NR1H4

유형Division-Arrested Cells Assay Kit

세포주293T (HEK)

System TypeGeneBLAzer™

Druggable TargetNuclear Receptors

Unit SizeEach