Thermo Scientific™

GeneBLAzer™ ER Beta DA Assay Kit

GeneBLAzer™ER beta DA(Division Arrested) cells and ER beta-UAS-bla GripTite™ 293 cells contain the ligand-binding domain (LBD) of the human Estrogen자세히 알아보기

GeneBLAzer™ER beta DA(Division Arrested) cells and ER beta-UAS-bla GripTite™ 293 cells contain the ligand-binding domain (LBD) of the human Estrogen receptor beta (ER beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla GripTite™ 293 cell line. GeneBLAzer™UAS-bla GripTite™ 293 cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-ER beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both ER beta DA cells and ER beta-UAS-bla GripTite™ 293 cells are functionally validated for Z` and EC50 concentrations of 17-beta Estradiol. In addition, ER beta-UAS-bla GripTite™ 293 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time. Additional testing data using alternate stimuli are also available.

사양

대체 작용제Cholic Acid, Deoxycholic Acid

길항제(z)-guggulsterone

어세이 입력Cell-based beta lactamase reporter gene

셀 상태Division-Arrested Cells

검출 방법Fluorescence

유전자 ID(Entrez)2100

유전자 기호ERβ

일차 작용제Chenodeoxycholic Acid (CDCA)

제품라인GeneBLAzer™

수량1 plate

리포터 유전자BLA (Beta-Lactamase)

타겟 입력ER beta, ESR2, ERB, NR3A2

세포주293T (HEK)

System TypeGeneBLAzer™

Druggable TargetNuclear Receptors

Unit SizeEach