Thermo Fisher Scientific Vinculin Polyclonal Antibody
Western blot과 IHC(P)에서 사용 가능한 Rabbit Polyclonal 항체로, Human, Mouse, Rat에 반응합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS 및 글리세롤 완충액에 보관됩니다. Vinculin 단백질 검출 및 세포 부착 관련 연구에 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human VCL. Recombinant protein control fragment (Product # RP-100519) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2789164 |
Product Specific Information
Immunogen sequence:
KMIDERQQEL THQEHRVMLV NSMNTVKELL PVLISAMKIF VTTKNSKNQG IEEALKNRNF TVEKMSAEIN EIIRVLQLTS WDEDAWASKD TEAMKRALAS IDSKLNQAKG WLRDPSASPG DAGEQAIRQI LDEAGKVGEL CAGKERREI
Target Information
Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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