Thermo Fisher Scientific CD20 Monoclonal Antibody (2H7)
CD20 단백질을 인식하는 마우스 단클론 항체로, Human 및 Non-human primate 시료에 반응합니다. IHC, Flow Cytometry, ICC/IF 등 다양한 응용에 사용 가능하며, Protein A로 정제된 액상 형태입니다. 4°C 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | Assay-Dependent | - |
| Flow Cytometry (Flow) | 1–4 µg/mL | View 3 publications |
| Immunoprecipitation (IP) | Assay-Dependent | - |
Product Specifications
| Category | Specification |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Published Species | Human, Non-human primate, Rhesus monkey |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2H7 |
| Immunogen | Human tonsillar B cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152399 |
Additional Conjugate Formats Available:
Product Specific Information
This antibody recognizes an extracellular epitope on CD20 (B1, Bp35), a 33–37 kDa non-glycosylated membrane receptor with four transmembrane domains. It is expressed on pre-B lymphocytes, resting and activated B cells (not plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.
Target Information
CD20 is a non-glycosylated surface phosphoprotein (33–37 kDa) expressed on mature and most malignant B cells, as well as on a subset of T lymphocytes and follicular dendritic cells. It regulates calcium conductance, cell cycle progression, and B-cell proliferation. CD20 is associated with lipid rafts and plays a role in BCR signaling. It remains on the cell surface after antibody binding, making it a key target for therapeutic B-cell depletion. Dysregulation of CD20 is linked to Ms4a1-related common variable immune deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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