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1 / 2Thermo Fisher Scientific F(ab`)2-Goat anti-Rabbit IgG (H+L) Secondary Antibody, FITC
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FITC 형광 표지된 염소 유래 항-토끼 IgG (H+L) 2차 항체로, IHC, ICC, Flow Cytometry 등 다양한 면역실험에 적합합니다. F(ab`)2 fragment 형태로 비특이적 결합을 최소화하며, 항원 친화 크로마토그래피로 정제되어 높은 특이성과 신뢰성을 제공합니다.
- 카탈로그번호
- 31579
- 판매단위
- pk
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Thermo Fisher Scientific 31579 F(ab`)2-Goat anti-Rabbit IgG (H+L) Secondary Antibody, FITC 1 mg pk판매 단위 pk ·
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Thermo Fisher Scientific · Thermo Fisher Scientific F(ab`)2-Goat anti-Rabbit IgG (H+L) Secondary Antibody, FITC
Thermo Fisher Scientific F(ab`)2-Goat anti-Rabbit IgG (H+L) Secondary Antibody, FITC
Applications and Tested Dilution
| Application | Dilution Range |
|---|---|
| Immunohistochemistry (IHC) | 1:50 - 1:200 |
| Immunocytochemistry (ICC/IF) | 1:50 - 1:200 |
| Flow Cytometry (Flow) | 1:50 - 1:200 |
| Immunoprecipitation (IP) | 1:50 - 1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rabbit |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Lyophilized |
| Concentration | 1.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.6, with 15 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_228236 |
| Target | IgG |
| Antibody Form | F(ab`)2 Fragment |
| Country of Origin | USA |
Product Specific Information
- Concentration may vary slightly from lot to lot; refer to datasheet for exact value.
- Product #31579 is suitable for Western blot, IF, ICC, IHC, IP, and FACS applications.
- Reacts with heavy chains of rabbit IgG and light chains common to most rabbit immunoglobulins; may cross-react with immunoglobulins from other species.
- Store protected from light at 4°C. For extended storage, add equal volume of glycerol (final 50%) and keep at -20°C.
- Fluorescein Amax = 492 nm; Emax = 520 nm.
- Fluorophore/Protein ratio: 10.3 µg/mg; ~2.5 moles FITC per mole F(ab`)2 (lot-dependent).
- Reconstitute with 0.75 mL distilled water (final 1.5 mg/mL).
Target Information
Anti-Rabbit secondary antibodies are affinity-purified with well-characterized specificity for rabbit immunoglobulins.
They are used for detection, sorting, or purification of rabbit IgG targets.
Secondary antibodies provide versatility and enhanced sensitivity through signal amplification.
Typically produced by immunizing the host with pooled rabbit immunoglobulins, followed by purification and conjugation to generate specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
Available Conjugate Formats
- Alexa Fluor 488 (10 nm, 5 nm colloidal gold)
- Alexa Fluor 568
- AP
- Biotin
- DyLight 350, 405, 488, 550, 594, 633, 650, 680, 800 4X PEG
- HRP
- NANOGOLD
- PE, PE-Cyanine5.5
- Qdot 525, 585, 605, 625, 655, 705, 800
- TRITC
- WesternDot 625
- Custom conjugation available upon request
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