Invitrogen™

ElectroMAX™ DH10B Cells

ElectroMAX DH10B Cells are electrocompetent E. coli cells offering the highest transformation efficiencies of >1 x 1010 cfu/μg plasmid DNA.자세히 알아보기

ElectroMAX DH10B Cells are electrocompetent E. coli cells offering the highest transformation efficiencies of >1 x 1010 cfu/μg plasmid DNA. The high transformation efficiencies make these cells ideal for cloning both prokaryotic and eukaryotic genomic DNA and efficient plasmid rescue from eukaryotic genomes.

ElectroMAX DH10B Cells are suitable for construction of gene banks, generation of cDNA libraries using plasmid-derrived vectors, and for situations when DNA is limited. They are ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction.

ElectroMAX DH10B Cells provide:
• High-efficiency transformation to maximize clones
• Enhanced genetic markers for cDNA or gDNA cloning capabilities

ElectroMAX DH10B Cell features
• Φ80_lac_ZΔM15 marker that provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-gal or Bluo-gal
• mcr_A genotypic marker and _mcr_BC, _mrr deletion that allow cloning DNA that contains methylcytosine and methyladenine
• _end_A1 mutation that enables increased plasmid yield and quantity

Genotype
F-mcr_A Δ(_mrr_-_hsd_RMS-_mcr_BC) Φ80_lac_ZΔM15 Δ_lac_X74 _rec_A1 _end_A1 _ara_D139Δ(_ara, leu)7697 gal_U _gal_K λ-_rps_L _nup_G
Get more detailed information about specific Genotypes and Genetic Markers of our _E. coli Competent Cells.

Find the strain and format that you need
DH10B cells are available in both electrocompetent and chemically competent formats.
ElectroMax DH10B T1R Cells and MegaX DH10B T1R Electrocomp Cells offer the benefit of T1 and T5 phage resistance.

Note: A high-voltage electroporation apparatus capable of generating field strengths of 16 kV/cm is required.

사양

제품 유형Electrocompetent Cells

에프에피솜 포함No

플라스미드 품질 개선Yes (endA1)

메틸화 DNA 클로닝Yes (mcrA)

형질전환 효율 수준High Efficiency (>1 x 109 cfu/μg)

항생제 내성 박테리아Yes (Streptomycin)

불안정 DNA 클로닝Not suitable for cloning unstable DNA

블루/화이트 스크리닝Yes (lacZΔM15)

고처리량 호환성Low

플라스미드May be used for plasmids >20 kb

비메틸화 DNA 준비No

재조합 감소Yes (recA1)

배송 조건Dry Ice

T1 Phage - 저항성(tonA)No

종E. coli (K12)

형식Tube

제품라인ElectroMAX™

수량5 x 100 μL

Unit SizeEach