Invitrogen™

MAX Efficiency™ DH5αF`IQ Competent Cells

MAX Efficiency DH5αF´IQ Competent Cells are high transformation efficiency chemically competent cells prepared by a patented modification of the procedure자세히 알아보기

MAX Efficiency DH5αF´IQ Competent Cells are high transformation efficiency chemically competent cells prepared by a patented modification of the procedure of Hanahan. This strain is a derivative of the DH5α strain, sharing the same benefits. The DH5αF´IQ strain contains an F´ episome and is a host for M13mp cloning vectors. It also supports cloning plasmids with an f1-like origin of replication (phagemids) or vector that uses expression from the lac promoter. DH5αF´IQ cells can yield >1 x 108 plaque forming units/μg M13mp19 RF with non-saturating amounts (5 pg) of DNA.

MAX Efficiency DH5αF´IQ Competent Cells have the _lac_ZΔM15 genotype, allowing blue-white screening on plates containing either X-Gal or Bluo-Gal. They also carry the _rec_A1 mutation which helps to reduce the rate of recombination while propagating plasmid DNA and the _end_A1 mutation that increases plasmid DNA yield and quantity.

MAX Efficiency DH5αF´IQ Competent Cells offer:
• >3 x 108 transformants/μg efficiency
• >1 × 108 plaque forming units/μg M13mp19 RF
• Stable F´ episome that eliminates need for growth on minimal media
• _lac_ZΔM15 marker that provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-Gal or Bluo-Gal
• Plasmid DNA maintenance with minimum recombination events due _rec_A1 mutation
• _end_A1 mutation that enables increased plasmid yield and quantity

Note: DH5αF`IQ lawn cells are provided to allow for plaque formation and a vial of monomer M13mp18 RF DNA are supplied as a control.

Genotype
F– φ80_lac_ZΔM15 Δ(lac_ZYA-_arg_F)U169 _rec_A1 _end_A1 _hsd_R17 (rK–, mK+) _pho_A _sup_E44 λ–_thi_–1 _gyr_A96 _rel_A1/F′ [_pro_AB+_lac_IqZΔM15 _zzf::_Tn_5(KmR)]

Find the strain and format that fits your needs
The DH5α strain and its derivatives are available with a variety of transformation efficiencies and in both electrocompetent and chemically competent formats.
DH5α-T1R competent cells are available in MultiShot format for high throughput applications.
Explore bacterial growth media formats.

사양

제품 유형Chemically Competent Cells

에프에피솜 포함Yes

플라스미드 품질 개선Yes (endA1)

메틸화 DNA 클로닝No

형질전환 효율 수준Medium Efficiency (1 x 108 to 1 x 109 cfu/μg)

항생제 내성 박테리아Yes (Kanamycin)

불안정 DNA 클로닝Not suitable for cloning unstable DNA

블루/화이트 스크리닝Yes (lacZΔM15)

고처리량 호환성Low

비메틸화 DNA 준비No

재조합 감소Yes (recA1)

배송 조건Dry Ice

T1 Phage - 저항성(tonA)No

종E. coli (K12)

프로모터Laclq

형식Tube

제품라인MAX Efficiency™

수량5 x 200 μL

Unit SizeEach