Thermo Fisher Scientific Phospho-RNA pol II CTD (Ser5) Recombinant Rabbit Monoclonal Antibody (POLR2A/9089R)

Applications

Tested Dilution

Publications

Western Blot (WB)

2-4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG, kappa

Expression System

CHO cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

POLR2A/9089R

Immunogen

Ten repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RNA pol II CTD,uniProtId:P24928-1,ncbiNodeId:9606,antigenRange:1615-1621,antigenLength:1970,antigenImageFileName:5430-RBM4-P0_RNA_pol_II_CTD_P24928-1_Rabbit.svg,antigenImageFileNamePDP:5430-RBM4-P0_RNA_pol_II_CTD_P24928-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

Protein A

Storage buffer

PBS with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Positive Control: HAP1 HepG2 K562 NIH3T3 or T47D cells. Human testis. PC3

Cellular Location: Nucleus.

Target Information

RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs. POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II. The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS. Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the CTD.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.