Thermo Fisher Scientific CD86 Monoclonal Antibody (C86, 3713)

Applications

Western Blot (WB)

• Tested Dilution: 1–2 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Product Specifications

Product Specific Information

Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. It acts as a costimulatory molecule during antigen presentation, providing T-cell help.
In the absence of sufficient co-stimulation involving CD86/CD80, tolerance may be induced. CD86 is a type I membrane protein belonging to the immunoglobulin superfamily, expressed by antigen-presenting cells.
It binds to CD28 and CTLA4 on T cells:

• CD86–CD28 interaction provides a costimulatory signal for T-cell activation.
• CD86–CTLA4 interaction negatively regulates T-cell activation, reducing immune response.

Alternative splicing produces multiple isoforms of CD86. Dysfunction of CD86 is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.