Thermo Fisher Scientific IRAK1 Polyclonal Antibody
IRAK1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. WB, ICC/IF, IP 등 다양한 응용에 적합하며, 인간·마우스·랫트 반응성. 항원 친화 크로마토그래피로 정제되었으며, 1 mg/mL 농도의 액상 형태로 제공.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 3 publications |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | - |
| Immunoprecipitation (IP) | 2–4 µg | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A peptide corresponding to amino acids near the carboxy terminus of human IRAK1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at -20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152551 |
Product Specific Information
- Suggested positive control: HeLa cell lysate
- PA5-19855 can be used with blocking peptide PEP-0001.
Target Information
IL-1 receptor associated kinases (IRAKs) are serine/threonine kinases involved in signaling via Toll/IL-1 pathways. IRAK1 is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Its association with MYD88 leads to phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation.
Additionally, IRAK1 can phosphorylate TIRAP to promote ubiquitination and degradation, and phosphorylates IRF7 to induce activation and nuclear translocation, resulting in transcriptional activation of type I IFN genes and antiviral responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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