Thermo Fisher Scientific CXCR4 Polyclonal Antibody
CXCR4 단백질을 인식하는 토끼 폴리클로날 항체로, Western blot 및 IHC(P) 실험에 적합합니다. 인간, 마우스, 랫트에 반응하며 항원 친화 크로마토그래피로 정제되었습니다. HeLa 세포 용해물을 양성 대조군으로 사용할 수 있습니다.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CXCR4 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL | - |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 15 amino acid peptide near the center of human CXCR4 isoform b |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage store at -20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153282 |
Product Specific Information
- A suggested positive control is HeLa cell lysate.
- PA5-19857 can be used with blocking peptide PEP-0002.
- The PA5-19857 immunogen is located within amino acids 170–220 of CXCR4.
- Predicted molecular weight: ~40 kDa.
- In Western blot applications, this antibody detects a band at 44 kDa (post-modification: 2 N-linked glycosylation).
- Predicted species reactivity based on immunogen sequence:
- Pig: 100%
- Bovine: 86%
- Sheep: 86%
- Mouse: 73%
- Rat: 67%
Target Information
CXCR4 (also known as Fusin, LESTR, HUMSTR) is a G-protein-coupled chemokine receptor with seven transmembrane domains. It functions as a co-receptor for X4 HIV-1 entry into CD4+ cells and also for HIV-2 via gp120-mediated fusion. CXCR4 acts as the receptor for the chemokine CXCL12/SDF-1, mediating intracellular calcium signaling and MAPK1/MAPK3 activation. It also binds extracellular ubiquitin, influencing calcium and cAMP levels.
CXCR4 plays critical roles in hematopoiesis, cardiac ventricular septum formation, and vascularization of the gastrointestinal tract through regulation of endothelial branching and remodeling. In the CNS, CXCR4 mediates hippocampal neuron survival and cerebellar development. It also binds bacterial LPS, mediating inflammatory responses including TNF secretion by monocytes. Antibodies against CXCR4 can block HIV-1 and HIV-2 fusion and infection of human target cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.