Thermo Fisher Scientific MDFI Polyclonal Antibody
Thermo Fisher Scientific의 MDFI Polyclonal Antibody는 인간, 마우스, 랫트 시료에서 MDFI 단백질을 검출합니다. Western blot 및 면역세포염색에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 고순도(>95%)를 보유하며 연구용으로 사용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 100–146 of human MDFI |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553604 |
Product Specific Information
- Detects endogenous MDFI protein at approximately 25 kDa.
- Purity >95% by SDS-PAGE.
Target Information
MDFI is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog (I-mf) show interference with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in vertebrae and rib formation, involving cartilage development. MDFI can also interfere with the DNA-binding activity of MyoD family members and plays roles in trophoblast and chondrogenic differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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