Thermo Fisher Scientific EGLN2 Monoclonal Antibody (OTI7H3), TrueMAB
EGLN2 단백질을 인식하는 마우스 단클론 항체로, Western blot과 IHC(파라핀 절편)에 적합합니다. 인간, 마우스, 랫트 반응성이 있으며, 동결건조 형태로 제공됩니다. 세포 내 산소 항상성 연구 및 HIF 경로 분석에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI7H3 |
| Immunogen | Full length human recombinant protein of human EGLN2 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Prolyl hydroxylase domain proteins HIF PHD1, HIF PHD2, and HIF PHD3 (also known as PHD1, PHD2, and PHD3 in rodents) hydroxylate HIF-alpha subunits.
Hypoxia-inducible factor (HIF) is a transcriptional regulator essential for oxygen homeostasis. The prolyl hydroxylases catalyze the posttranslational formation of 4-hydroxyproline in HIF-alpha proteins.
HIF PHD1, encoded by EGLN2 (chromosome 19q13.3), is widely expressed with highest levels in testis and functions as a cellular oxygen sensor, playing a key role in cell growth regulation. It can localize to both the nucleus and cytoplasm and is detected in hormone-responsive tissues such as mammary, ovarian, and prostate epithelium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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