Thermo Fisher Scientific SMAD5 Monoclonal Antibody (ZS004)
SMAD5 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, 인간·마우스·랫트에 반응합니다. Western blot, IHC, ICC, ELISA 등 다양한 실험에 사용 가능하며, Protein A로 정제된 액상 형태입니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:100 | - |
| Immunocytochemistry (ICC/IF) | 1 µg/mL | - |
| ELISA | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ZS004 |
| Immunogen | Recombinant protein derived from an internal region of the human and rat Smad5 proteins |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533420 |
Target Information
SMADs are members of the MAD-related family of molecules. These intracellular proteins are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta (TGF-β) superfamily.
SMADs are classified into:
- Receptor-regulated SMADs (R-SMADs): SMAD1, 2, 5, 8, and 9
- Common-mediator SMAD (co-SMAD): SMAD4
- Inhibitory SMADs (I-SMADs): SMAD6 and 7
SMAD1, 5, 8, and 9 share high sequence homology, and antibodies recognizing common sequences among them are available. SMAD8 and SMAD9 are often used interchangeably in literature.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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