Takara Fluorescent labeling and transfection control

These vectors are ideally suited as controls to measure transfection efficiency. Simply co-transfect a red fluorescent protein vector with the target plasmid, and the resulting cells that are labeled with the fluorescent protein will also likely be transfected with the experimental plasmid. Each vector expresses a fluorescent protein from a constitutive promoter (CMV or EF1-alpha) and, if required, stable transfected cells can be selected for using G418.

These vectors are ideally suited as controls to measure transfection efficiency. Simply co-transfect a red fluorescent protein vector with the target plasmid, and the resulting cells that are labeled with the fluorescent protein will also likely be transfected with the experimental plasmid. Each vector expresses a fluorescent protein from a constitutive promoter (CMV or EF1-alpha) and, if required, stable transfected cells can be selected for using G418. Transfected cells can also be used in assays that require fluorescent labeling of whole cells.

These vectors do not contain a multiple cloning site; other fluorescent protein vectors are available for cloning applications. Click here for green fluorescent protein options.

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Overview

• Bright fluorescent proteins for labeling transfected cells

• Choice of CMV or EF1-alpha promoter

  • CMV promoter for the strongest transcriptional activity in the broadest range of cell types
  • Human elongation factor-1 alpha (EF1-alpha) promoter that can be used in cells where CMV can be silenced (e.g., embryonic stem cells and some hematopoietic cells)

• Multiple red color options

  • tdTomato is the brightest of the red fluorescent proteins and is also extremely suitable for in vivo imaging applications
  • E2-Crimson, a far-red fluorescent protein, is bright, very soluble, and matures extremely fast (half time of 26 minutes at 37°C). It exhibits low cytotoxicity and is well-suited for in vivo applications involving sensitive cells such as primary cells and stem cells.

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Applications

• Fluorescent reporter for transient and stable transfections
• Whole-cell labeling

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.