Thermo Fisher Scientific BCKDHB Recombinant Rabbit Monoclonal Antibody (5C7B3)
BCKDHB 단백질을 인식하는 토끼 단클론 항체로, Western blot과 ELISA에 적합합니다. HEK293 세포에서 발현된 재조합 항체이며, Protein A로 정제되었습니다. PBS 기반 버퍼에 저장되며 -20°C에서 보관합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000–1:12,000
ELISA
- Tested Dilution: 1 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 5C7B3 |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 51–392 of human BCKDHB |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.44 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
Product Specific Information
Immunogen Sequence:
VAHFTFQPDP EPREYGQTQK MNLFQSVTSA LDNSLAKDPT AVIFGEDVAF GGVFRCTVGL RDKYGKDRVF NTPLCEQGIV GFGIGIAVTG ATAIAEIQFA DYIFPAFDQI VNEAAKYRYR SGDLFNCGSL TIRSPWGCVG HGALYHSQSP EAFFAHCPGI KVVIPRSPFQ AKGLLLSCIE DKNPCIFFEP KILYRAAAEE VPIEPYNIPL SQAEVIQEGS DVTLVAWGTQ VHVIREVASM AKEKLGVSCE VIDLRTIIPW DVDTICKSVI KTGRLLISHE APLTGGFASE ISSTVQEECF LNLEAPISRV CGYDTPFPHI FEPFYIPDKW KCYDALRKMI NY
Target Information
Branched-chain keto acid dehydrogenase (BCKD) is a multienzyme complex located in the inner mitochondrial membrane, functioning in the catabolism of branched-chain amino acids. The complex includes three components:
- E1 (branched-chain alpha-keto acid decarboxylase)
- E2 (lipoamide acyltransferase)
- E3 (lipoamide dehydrogenase)
This gene encodes the E1 beta subunit. Mutations in this gene are associated with Maple Syrup Urine Disease (MSUD), type 1B, which is characterized by a maple syrup odor in urine, mental and physical retardation, and feeding problems.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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