Applied Biosystems™

SYBR™ Select Master Mix for CFX

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we`ve taken the best of SYBR Select Master Mix for CFX and added additional capabilities for your gene expression analysis.

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SYBR™ Select Master Mix for CFX is a superior and cost-effective solution for your real-time PCR applications.

• Specific—minimize primer-dimer and non-specific amplification
• Reproducible and sensitive—consistent amplification across a wide dynamic range
• Bright—contains SYBR™ GreenER™ dye for maximum brightness
• Carry-over contamination control—contains heat-labile UDG
• For use with the Bio-Rad CFX96™ Touch or CFX384™ Touch systems
• Standard and fast cycling parameters

Formulated for Maximum Specificity
SYBR™ Select Master Mix for CFX contains all the components needed for your real-time PCR reactions, except the template and primers, in a convenient 2X concentration premix. The master mix includes AmpliTaq™ DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity.

Obtain Reproducible Results Across a Wide Dynamic Range
SYBR™ Select Master Mix for CFX is specially formulated to provide robust results from 100 ng to 0.1 pg cDNA per reaction (Figure 1) and can reliably detect a single copy of genomic DNA (Figure 2).

Contains SYBR™ GreenER™ Dye and Heat-labile UDG
• SYBR™ GreenER™ dye is less inhibitory to PCR than SYBR™ Green I dye, resulting in brighter signals
• Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control

View performance data.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

사양

검출 방법SYBR

형태Liquid

형식Vial

GC-Rich PCR PerformanceHigh

PCR 방법qPCR

중합효소AmpliTaq DNA Polymerase

반응 속도Fast or Standard

용도(장비)AB 7900HT, Standard Mode, AB StepOne™, Fast Mode, AB 7500, Standard Mode, AB 7500, Fast Mode, Bio-Rad CFX96 Touch™, AB StepOnePlus™, Fast Mode, AB 7900HT, Fast Mode, AB StepOne™, Standard Mode, Bio-Rad CFX Connect™, AB 7900 HT 384-Well System, Bio-Rad CFX384 Touch™, AB StepOnePlus™, Standard Mode

라벨 또는 염료SYBR GreenER

패시브 참조 염료ROX (Pre-mixed)

제품라인SYBR™

제품 유형Real Time PCR SYBR Master Mix

순도 또는 품질 등급UP (Ultra Pure)

수량1 x 1 mL

배송 조건Wet Ice

충분100 Reactions of 20 μL

용도(애플리케이션)Gene Expression

농도2X

핫 스타트Built-In Hot Start

반응 수100 Reactions

샘플 종류DNA (Genomic), cDNA

부피1 mL

Unit SizeEach