Applied Biosystems™

SYBR™ Select Master Mix

SYBR™ Select Master Mix is a superior and cost-effective solution for your real-time PCR applications.Features of the SYBR™ Select Master자세히 알아보기

SYBR™ Select Master Mix is a superior and cost-effective solution for your real-time PCR applications.

Features of the SYBR™ Select Master Mix include:

• Specific—minimize primer-dimer and non-specific amplification
• Reproducible and sensitive—consistent amplification across a wide dynamic range
• Bright—contains SYBR™ GreenER™ dye for maximum brightness
• Carry-over contamination control—contains heat-labile UDG
• Can be used in either standard or fast cycling mode

Formulated for Maximum Specificity
SYBR™ Select Master Mix contains all the components needed for your real-time PCR reaction, except the template and primers, in a convenient 2X concentration premix. The master mix includes AmpliTaq™ DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity.

Obtain Reproducible Results Across a Wide Dynamic Range
SYBR Select Master Mix is specially formulated to provide robust results at 100 ng to 0.1 pg cDNA per reaction (Figure 1) and can reliably detect a single copy of genomic DNA (Figure 2).

Contains SYBR™ GreenER™ Dye and Heat-labile UDG
• SYBR™ GreenER™ dye is less inhibitory to PCR than SYBR™ Green I dye resulting in brighter signals
• Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control

Instrument Compatibility
SYBR™ Select Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments except the 7900HT real-time PCR system. It is also compatible with the Bio-Rad IQ™5, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems.

View performance data.

Alternative product
Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we`ve taken the best of SYBR Select Master Mix and added additional capabilities for your gene expression analysis.

사양

검출 방법SYBR

형태Liquid

형식Vial

GC-Rich PCR PerformanceHigh

PCR 방법qPCR

중합효소AmpliTaq DNA Polymerase

반응 속도Fast or Standard

용도(장비)7500 Fast System, 7500 System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Fast Mode, StepOne™, Standard Mode, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System, AB StepOnePlus™, Fast Mode, AB StepOnePlus™, Standard Mode, AB 7500, Fast Mode, AB StepOne™, Fast Mode, AB StepOne™, Standard Mode

라벨 또는 염료SYBR GreenER

패시브 참조 염료ROX (Pre-mixed)

제품라인SYBR™

제품 유형Real Time PCR SYBR Master Mix

순도 또는 품질 등급UP (Ultra Pure)

수량1 x 1 mL

배송 조건Wet Ice

충분100 Reactions of 20 μL

용도(애플리케이션)Gene Expression

농도2X

핫 스타트Built-In Hot Start

반응 수100 Reactions

샘플 종류DNA (Genomic), cDNA

부피5 mL

Unit SizeEach