Thermo Fisher Scientific SSEA3 Monoclonal Antibody (MC-631), DyLight 488
인간 세포에서 SSEA3 검출용 DyLight 488 결합 단일클론 항체. ICC/IF, 유세포분석, ELISA에 적합. 492/519 nm 형광 특성. 4°C 보관, 동결 금지. 연구용 전용 시약.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:10–1:100 |
| Flow Cytometry (Flow) | 1:50–1:500 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rat / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MC-631 |
| Immunogen | Fischer rat immunized with 4–8 cell stage mouse embryos |
| Conjugate | DyLight™ 488 |
| Excitation / Emission Max | 492 / 519 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography - MBP |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536683 |
Available Formats
Product Specific Information
MA1-020-D488 has been successfully used in ICC/IF and flow cytometry applications on human samples.
It can be used for immunofluorescence analysis of SSEA3 in human induced pluripotent stem cells (iPSCs).
Target Information
SSEA-3 (stage-specific embryonic antigen 3) is a cell surface antigen expressed in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPSCs), along with SSEA-4, TRA-1-60, and TRA-1-81.
In human cells, these markers are down-regulated during differentiation, while SSEA-1 is absent in undifferentiated human stem cells and upregulated after retinoic acid-mediated differentiation.
In mouse stem cells, SSEA-1 expression decreases with differentiation, whereas SSEA-3 and SSEA-4 expression increase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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