Thermo Fisher Scientific N-cadherin Monoclonal Antibody (5D5)
N-cadherin 단백질을 검출하는 Mouse monoclonal antibody로, Human, Mouse, Rat 시료에 반응합니다. Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 응용에 적합합니다. CDH2 단백질 연구 및 세포 부착 관련 연구에 활용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 7 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 | - |
| Flow Cytometry (Flow) | 1:200–1:400 | - |
| ELISA | 1:10,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5D5 |
| Immunogen | Purified recombinant fragment of human CDH2 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_10985437 |
Product Specific Information
MA5-15633 targets CDH2 in indirect ELISA, FACS, IF, IHC, and WB applications, showing reactivity with Human, Mouse, and Rat samples.
The immunogen is a purified recombinant fragment of human CDH2 expressed in E. coli.
MA5-15633 detects CDH2 with a predicted molecular weight of approximately 99.8 kDa.
Target Information
N-cadherin is a 140 kDa transmembrane protein mediating calcium-dependent intercellular adhesion. It belongs to the cadherin family, which regulates morphogenetic movements and cell surface adhesion through homotypic interactions.
Its function depends on association with the actin cytoskeleton via interactions between the C-terminal region of N-cadherin and cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin.
N-cadherin is a calcium-dependent cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail. It functions during gastrulation and is essential for establishing left-right asymmetry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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