Thermo Fisher Scientific NEFM Polyclonal Antibody
Rabbit polyclonal antibody targeting NEFM (Neurofilament Medium). Suitable for WB, IHC (Paraffin/Frozen), and ICC/IF applications. Reacts with multiple species. Provided as liquid whole serum without preservative. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:1,000–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Avian, Bovine, Cat, Human, Mammal, Mouse, Pig, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant C-terminal segment of rat NF-M |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1956148 |
Product Specific Information
PA1-32348 detects NEFM from rat samples.
Target Information
Neurofilaments (NFs) are intermediate filaments expressed almost exclusively in neuronal cells, predominantly in large axons. They consist of three polypeptide chains—NF-M, NF-H, and NF-L—that share structural similarity in a coiled-coil core domain but differ in terminal sequences. The C-terminal tails of NF-M and NF-H mediate filament interactions, influencing axonal size and cytoskeletal architecture. NF-M phosphorylation and O-GlcNAcylation regulate its function and translocation. Antibodies against neurofilament subunits serve as robust neuronal markers due to their abundance, neuron-specific expression, and biochemical stability.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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