
Thermo Fisher Scientific KLF4 Recombinant Rabbit Monoclonal Antibody (BLR250L)
KLF4 단백질을 표적하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC/IF, IP 등 다양한 응용에 적합함. 인간 및 생쥐 반응성, HEK293F 발현 시스템 사용. 고순도 정제, 1 mg/mL 농도, 연구용 전용.
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Thermo Fisher Scientific KLF4 Recombinant Rabbit Monoclonal Antibody (BLR250L)
Applications and Tested Dilutions
- Western Blot (WB): 1:1,000
- Immunohistochemistry (Paraffin) (IHC (P)): 1:100–1:500
- Immunocytochemistry (ICC/IF): 1:100–1:500
- Immunoprecipitation (IP): 6 µL/mg of lysate
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR250L |
| Immunogen | Peptide representing a region between residue 75 and 125 of Human Krueppel-like factor 4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 8.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunocytochemistry: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
Target Information
Kruppel-like factor 4 (KLF4) protein is encoded by the KLF4 gene located on chromosome 9 in humans. Also known as gut-enriched Kruppel-like factor (GKLF) or epithelial zinc-finger protein (EZF), it belongs to the Kruppel-like factor family of DNA-binding zinc finger transcription factors.
KLF4 plays a key role in cellular processes such as endothelial signaling, adipogenesis, differentiation, embryogenesis, and cell cycle control. As one of the four Yamanaka factors, it is crucial for reprogramming differentiated cells into pluripotent stem cells.
Its localization determines biological significance—nuclear localization is associated with oncogenic function, while cytosolic localization correlates with tumor suppression effects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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