Thermo Fisher Scientific HIST1H2BH Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to the N-terminal of Human HIST1H2BH. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HIST1H2BH,uniProtId:Q93079-1,ncbiNodeId:9606,antigenRange:1,antigenLength:126,antigenImageFileName:PA5-75322_HIST1H2BH_Q93079-1_Rabbit.svg,antigenImageFileNamePDP:PA5-75322_HIST1H2BH_Q93079-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2719050

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Target Information

In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B H3 and H4 and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. Histone H1 is required for the condensation of nucleosome chains into higher order structures. Phosporylation of Histone H1 is thought be involved in this process, although the exact nature of this role has yet to be elucidated. Evidence suggests that Histone H1 is a part of a general repressor mechanism for stable repression of transcription, but it can also activate transcription of specific genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.