Thermo Fisher Scientific UAP1L1 Polyclonal Antibody
Thermo Fisher Scientific의 UAP1L1 폴리클로날 항체로, Western blot, IHC, ICC/IF 등에 적합합니다. 인간 유래 단백질에 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. 4°C 단기 보관, -20°C 장기 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human UAP1L1. Recombinant protein control fragment (Product # RP-93117) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2649161 |
Product Specific Information
Immunogen sequence:
SPNWTLDPEP RCRLWSEPRL PAGPGVLAAG SPRLPCRYVM TSEFTLGPTA EFFREHNFFH LDPANVVMFE QRLLPAVTFD GKVILER
Sequence identity:
Highest antigen sequence identity to the following orthologs:
- Mouse: 46%
- Rat: 47%
Target Information
UAP1 is a 56 kDa isoform that catalyzes the final step of UDP-N-acetylglucosamine (UDP-GlcNAc) biosynthesis from fructose-6-phosphate. UDP-GlcNAc, the activated form of the GlcNAc moiety, is a key precursor in N-linked glycosylation and the glycosylphosphatidylinositol (GPI)-anchor of cellular proteins.
Crystallization studies revealed that UAP1 is composed of a large central core flanked by two smaller extra domains. The main domain displays an alpha/beta structure resembling the Rossmann fold.
Northern blot analysis detected abundant expression of UAP1 in testis and sperm, with lower expression in placenta, muscle, and liver. It is implicated in antibody-mediated infertility in males.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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