Thermo Fisher Scientific p14ARF Polyclonal Antibody
인간 p14ARF 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 IHC에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 액상 형태로 제공됩니다. 4°C에서 보관하며, 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,500 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 82 and the C-terminus (residue 132) of human p14ARF |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Phosphate/Tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
The INK4a locus on chromosome 9p21 is frequently affected in human tumors. It encodes two structurally distinct tumor suppressor proteins, p16INK4a and the alternative reading frame protein, ARF (p14ARF in human and P19ARF in mouse).
These two proteins interact with upstream tumor suppressors, retinoblastoma protein and p53, respectively. Each protein plays a role in cellular senescence, cell cycle regulation, and tumor suppression.
The p14ARF (or P19ARF in mouse) proteins can induce both G1 and G2 phase arrest in a p53-dependent manner. Functional differences between mouse and human ARF proteins may contribute to varying tumor susceptibilities across species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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