Thermo Fisher Scientific HTR1B (extracellular) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:400

View 1 publication 1 publication

Immunohistochemistry (IHC)

1:200

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Product Specifications

Published species

Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide corresponding to amino acid residues 33-49 of human 5-Hydroxytryptamine receptor 1B (Extracellular, N-terminus) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HTR1B,uniProtId:P28222-1,ncbiNodeId:9606,antigenRange:34-49,antigenLength:390,antigenImageFileName:PA5-111905_HTR1B_P28222-1_Rabbit.svg,antigenImageFileNamePDP:PA5-111905_HTR1B_P28222-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.8 mg/mL

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2857313

Product Specific Information

Reconstitute antibody with 50 µL of double distilled water (DDW). The reconstituted solution can be stored at 4 degrees C for up to 1 week. For longer periods, small aliquots should be stored at -20 degrees C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Peptide sequence is: CSAKDYIYQD SISLPWK

Homology: Rat - 15/17 amino acid residues identical; mouse - 14/17 amino acid residues identical.

Target Information

Serotonin (5-hydroxytryptamine, 5-HT), originally discovered as a serum factor plays important roles in regulating diverse biological processes in central and peripheral nervous systems, cardiovascular systems, and gastrointestinal systems. Molecular cloning studies have identified 5 receptors belonging to the 5-HT1 subtypes. These receptors are negatively coupled to adenylyl cyclase. Radioligand binding studies suggest the presence of 5-HT1B (formerly known as 5-HT1DbR) sites in substantia nigra, dorsal subiculum, globus pallidus, and superior collicum. In situ hybridization studies show abundance of 5-HT1BR mRNA in CA1 pyramidal cells of hippocampus, Purkinje cells of cerebellum, olfactory tubercle, subthalamic nucleus and somewhat lower levels in the caudate-putamen, cortical areas, thalamic and hypothalamic nuclei.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.