Thermo Fisher Scientific Cytokeratin 10/13 Monoclonal Antibody (DE-K13)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1-2 µg/mL

-

Immunocytochemistry (ICC/IF)

1-2 µg/mL

-

Flow Cytometry (Flow)

1-2 µg/10^6 cells

-

Product Specifications

Species Reactivity

Cat, Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

DE-K13

Immunogen

Cytoskeletal preparation extracted from human ectocervical epithelium if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cytokeratin 10/13,uniProtId:P13645-1,ncbiNodeId:9606,antigenRange:1-584,antigenLength:584,antigenImageFileName:MSM11-696-P1ABX_Cytokeratin_1013_P13645-1_House_mouse.svg,antigenImageFileNamePDP:MSM11-696-P1ABX_Cytokeratin_1013_P13645-1_House_mouse_PDP.jpeg,sortOrder:1},{targetFamily:Cytokeratin 10/13,uniProtId:P13646-1,ncbiNodeId:9606,antigenRange:1-458,antigenLength:458,antigenImageFileName:MSM11-696-P1ABX_Cytokeratin_1013_P13646-1_House_mouse.svg,antigenImageFileNamePDP:MSM11-696-P1ABX_Cytokeratin_1013_P13646-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

Cytokeratins(CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie. Skin) and non-keratinizing cells (ie. Mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different CKs in the molecular range of 40-70 k Da and isoelectric points of 5-805 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.