Thermo Fisher Scientific Cytokeratin 10/13 Monoclonal Antibody (DE-K13)
Cytokeratin 10/13 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 사용 가능. 고순도 Protein A/G 정제, 보존제 무첨가 PBS 용액 형태로 제공. 연구용 전용 제품.
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- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Cat, Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | DE-K13 |
| Immunogen | Cytoskeletal preparation extracted from human ectocervical epithelium |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Cytokeratins (CK) are intermediate filaments of epithelial cells found in both keratinizing tissues (e.g., skin) and non-keratinizing cells (e.g., mesothelial cells). Although not typical markers for endothelial cells, cytokeratins have been detected in some microvascular endothelial cells. At least 20 different CKs, ranging from 40–70 kDa with isoelectric points of 5–8, can be identified by two-dimensional gel electrophoresis.
CKs are classified into two major types: Type I (acidic polypeptides) and Type II (basic polypeptides). Typically, epithelial cells express at least one member of each type.
Monoclonal antibodies targeting cytokeratin proteins are valuable tools for tumor identification and classification.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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