Thermo Fisher Scientific MAPK7 Monoclonal Antibody (OTI3H2), TrueMAB
인간 MAPK7(ERK5)을 인식하는 Mouse IgG1 단일클론 항체로, WB, ICC/IF, Flow Cytometry에 적합합니다. 무보존제, Lyophilized 형태로 제공되며, 재구성 후 1 mg/mL 농도로 사용 가능합니다. 세포 신호전달 및 분화 연구용으로 최적화된 TrueMAB 제품입니다.
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Thermo Fisher Scientific MAPK7 Monoclonal Antibody (OTI3H2), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3H2 |
| Immunogen | Full-length human recombinant protein of MAPK7 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
MAPK7 (ERK5) is a serine/threonine kinase belonging to the MAP kinase family, involved in cellular processes such as proliferation, differentiation, transcription regulation, and development.
Activated by MAP2K5/MEK5, MAPK7 translocates to the nucleus in response to extracellular signals to regulate gene expression through phosphorylation of transcription factors.
Gene deletion in mice leads to defective cardiovascular development and embryonic lethality.
ERK5 is expressed in adult tissues including heart, placenta, lung, kidney, and skeletal muscle, but not in liver.
Four alternatively spliced transcript variants encoding two distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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