Thermo Fisher Scientific PHF7 Monoclonal Antibody (OTI1B4), TrueMAB
PHF7 단백질을 인식하는 마우스 단클론 항체로, Western blot, ICC/IF, Flow cytometry에 적합. Human 시료 반응성, 비결합형 형태로 고순도 정제. 동결건조 상태로 제공되며, 재구성 후 1 mg/mL 농도로 사용 가능. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1B4 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 155–381 of human PHF7 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, it is recommended to perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Spermatogenesis is a complex process regulated by extracellular and intracellular factors and by interactions among testicular interstitial cells, Sertoli cells, and germ cells. This gene is expressed in Sertoli cells but not in germ cells. Lack of expression has been observed in patients with spermatogenic arrest at the spermatocyte stage, a condition that can lead to oligospermia or azoospermia.
The encoded proteins contain plant homeodomain (PHD) finger domains, also known as leukemia-associated protein (LAP) domains, which are believed to be involved in transcriptional regulation. This protein localizes to the nucleus of transfected cells and is implicated in the transcriptional regulation of spermatogenesis. Two isoforms are produced by alternative transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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