Thermo Fisher Scientific HRAS Monoclonal Antibody (OTI1F2), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).

Target Information

Monomeric G protein Ras functions as a molecular switch linking receptor and nonreceptor tyrosine kinase activation to downstream signaling pathways that lead to distinct cytoplasmic or nuclear events.
Each mammalian cell contains three Ras proto-oncogenes encoding closely related Ras proteins: H-, K-, and N-Ras.
Oncogenic mutations in Ras genes are found in approximately 30% of all human cancers. Constitutive activation of Ras due to mutations or overexpression promotes proliferation and inhibits apoptosis.

• K-Ras mutations: common in pancreatic, colorectal, and non-small-cell lung carcinomas
• H-Ras mutations: common in bladder, kidney, and thyroid carcinomas
• N-Ras mutations: found in melanoma, hematological malignancies, and hepatocellular carcinoma

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.