Thermo Fisher Scientific B3GNT8 Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:20–1:50

Product Specifications

Product Specific Information

Immunogen sequence:
TPANPEPTLP ANLSTRLGQT IPLPFAYWNQ QQWRLGSLPS GDSTETGGCQ AWGAAAATEI PDFASYPKDL RRFLLSAACR SFPQWLP

Highest antigen sequence identity to the following orthologs:

• Mouse: 69%
• Rat: 69%

Target Information

B3GNT8 is a galactosyltransferase involved in the synthesis of poly-N-acetyllactosamine (polyLacNAc), a linear chain of repeating LacNAc units composed of galactose (Gal) and N-acetylglucosamine (GlcNAc). The B3GNT8 gene is mapped to chromosome 19q13.2.

A soluble form of B3GNT8 overexpressed in HEK293 cells selectively transferred GlcNAc from UDP-GlcNAc to the nonreducing terminus of Gal-beta-1-4-GlcNAc-alpha-p-nitrophenyl phosphate and lactoside-alpha-benzoyl. It did not utilize keratan sulfates or polylactosamine oligosaccharide as substrates. B3GNT8 activity required Mn²⁺ and was less efficient with Co²⁺. The pH optimum was between 7 and 7.5.

B3GNT8 also transferred GlcNAc onto alpha-1-acid glycoprotein and ovomucoid, which possess tetraantennary and pentaantennary complex type N-glycans. With a tetraantennary N-glycan substrate, B3GNT8 appeared to prefer the beta-1-2 branch over the beta-1-6 branch. When overexpressed in HCT15 human colon cancer cells, B3GNT8 increased cell surface expression of both polyLacNAc and beta-1-6-branched N-glycans.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.