Thermo Fisher Scientific NEK9 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 730-979 of human NEK9 (NP_149107.4). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NEK9,uniProtId:Q8TD19-1,ncbiNodeId:9606,antigenRange:730-979,antigenLength:979,antigenImageFileName:PA5-120584_NEK9_Q8TD19-1_Rabbit.svg,antigenImageFileNamePDP:PA5-120584_NEK9_Q8TD19-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.79 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914156

Product Specific Information

Positive test controls include: HepG2, Raji, Mouse heart, Rat thymus, Rat lung, Rat spleen.

Immunogen sequence: SKTIRSNSSG LSIGTVFQSS SPGGGGGGGG GEEEDSQQES ETPDPSGGFR GTMEADRGME GLISPTEAMG NSNGASSSCP GWLRKELENA EFIPMPDSPS PLSAAFSESE KDTLPYEELQ GLKVASEAPL EHKPQVEASS PRLNPAVTCA GKGTPLTPPA CACSSLQVEV ERLQGLVLKC LAEQQKLQQE NLQIFTQLQK LNKKLEGGQQ VGMHSKGTQT AKEEMEMDPK PDLDSDSWCL LGTDSCRPSL

Target Information

NEK9, a NEK type protein kinase, regulates chromosome alignment and segregation in mitosis. The protein has a N-terminal NIMA-like catalytic domain, a central domain with homology to the guanine nucleotide exchange factor for the GTPase Ran (RCC1), and a C-terminal coiled-coil domain. It is phosphorylated by active p34(Cdc2) and is capable of autophosphorylation and oligomerization. The activated protein binds Nek6 and RCC1, and has been shown to associate with the human homolog of the Drosophila protein Bicaudal D (Bicd2) in vivo and to phosphorylate this coiled-coil protein in vitro. NEK9 expression has been documented in normal human brain. ESTs have been isolated from several tissue libraries, including normal bone, lymph and thymus and cancerous cervix, eye, lymph and placenta.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.