Thermo Fisher Scientific CNOT4 Monoclonal Antibody (OTI1H2)
CNOT4 단백질을 인식하는 Mouse Monoclonal Antibody. Western blot 및 IHC(P) 검증 완료. 인간 유래 단백질(aa 190-455)을 면역원으로 사용. 1 mg/mL 농도의 액상 제품으로, PBS/BSA/glycerol buffer에 보관. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1H2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 190–455 of CNOT4 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2724167 |
Target Information
CNOT4 is a 575 amino acid protein with E3 ubiquitin ligase activity, mainly involved in protein modification and ubiquitination. It contains a RING-type zinc finger domain (C4C4 type), an RNA recognition motif, and a bipartite nuclear localization signal.
CNOT4 is a component of the CCR4-NOT (CAF1) core complex that regulates transcription of multiple genes, exerting both positive and negative effects on gene expression. It interacts with CNOT1 via the C-terminus and with E2 ubiquitin ligases via the C4C4 RING domain. Reports suggest that CNOT4 becomes phosphorylated in response to DNA damage at consensus sites recognized by ATM and ATR.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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