Thermo Fisher Scientific TPRKB Monoclonal Antibody (OTI3E3)
TPRKB 단백질을 인식하는 마우스 단클론 항체로 Western blot, IHC, Flow Cytometry에 사용 가능. 고순도의 액상 포맷으로 제공되며, PBS/BSA/glycerol 완충액에 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3E3 |
| Immunogen | Full length human recombinant protein of TPRKB produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2725549 |
Target Information
TPRKB is a component of the EKC/KEOPS complex required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. The complex is likely involved in transferring the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. TPRKB acts as an allosteric effector regulating the t6A activity of the complex but is not required for tRNA modification.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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