Thermo Fisher Scientific EIF2S1 Recombinant Rabbit Monoclonal Antibody (PSH04-29)
EIF2S1 단백질을 표적으로 하는 Thermo Fisher Scientific의 재조합 토끼 단클론 항체. WB, IHC, ICC/IF, Flow Cytometry에 적용 가능. HEK293 발현 시스템, Protein A 정제, PBS/glycerol 저장. 인체 및 설치류 반응성, 스트레스 관련 세포 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PSH04-29 |
| Immunogen | Recombinant protein within human EIF2S1 aa 1–315/315 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.1% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
Product Specific Information
- Positive Control: MCF7, HepG2, HeLa, COS-1, A549, RAW264.7, C6 cell lysates; mouse and rat kidney/spleen tissue lysates; human, mouse, and rat breast tissue.
- Sequence Similarities: 99% Mouse/Rat
- Subcellular Location: Cytoplasm, Stress granule
- Predicted Band Size: 36 kDa
Target Information
EIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of alpha, beta, and gamma subunits. This translation initiation factor facilitates binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex. EIF2A can be phosphorylated by stress-activated kinases, and phosphorylated EIF2A inhibits EIF2B activity, preventing guanine nucleotide exchange.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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