Thermo Fisher Scientific EIF2S1 Recombinant Rabbit Monoclonal Antibody (PSH04-29)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

-

Flow Cytometry (Flow)

1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

PSH04-29

Immunogen

Recombinant protein within human eif2s1 aa 1-315/315. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EIF2S1,uniProtId:P05198-1,ncbiNodeId:9606,antigenRange:1-315,antigenLength:315,antigenImageFileName:MA5-52465_EIF2S1_P05198-1_Rabbit.svg,antigenImageFileNamePDP:MA5-52465_EIF2S1_P05198-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.1 BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

Product Specific Information

Positive Control: MCF7 cell lysate, HepG2 cell lysate, HeLa cell lysate, COS-1 cell lysate, A549 cell lysate, RAW264.7 cell lysate, C6 cell lysate, mouse kidney tissue lysate, mouse spleen tissue lysate, rat kidney tissue lysate, rat spleen tissue lysate, HepG2, human breast tissue, mouse breast tissue, rat breast tissue.

Sequence Similarities: 99% Mouse/Rat.

Subcellular Location: Cytoplasm, Stress granule.

Predicted band size: 36 kDa.

Target Information

EIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex. The polypeptide can be phosphorylated by related protein kinases activated in response to stress. Phosphorylated EIF2A inhibits EIF2B activity and prevents guanine nucleotide exchange.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.