Thermo Fisher Scientific ATG4B Polyclonal Antibody
ATG4B 단백질을 인식하는 Thermo Fisher Scientific의 Sheep Polyclonal 항체. Western blot 및 IHC에 사용 가능. 인간 및 생쥐 반응성. 동결건조 형태로 제공되며, 4°C 또는 -20°C에서 보관. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:200–1:2,000 | – |
| Immunohistochemistry (IHC) | 1:200–1:2,000 | – |
| Miscellaneous PubMed (Misc) | – | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Sheep / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from human ATG4B conjugated to an immunogenic carrier protein has been used as the antigen |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 150 µL of sterile water.
- Centrifuge to remove any insoluble material.
- Specificity: ATG4B.
Target Information
Cysteine protease ATG4B is an enzyme encoded by the ATG4B gene. Autophagy is a critical cellular homeostatic process that controls the turnover of damaged organelles and proteins. ATG4B is a deubiquitin-like protease that plays dual roles in the core machinery of autophagy. It cleaves the C-terminal amino acid of ATG8 family proteins (MAP1LC3, GABARAPL1, GABARAPL2, and GABARAP) to reveal a C-terminal glycine. Exposure of this glycine is essential for conjugation of ATG8 proteins to phosphatidylethanolamine (PE) and membrane insertion, which is necessary for autophagy. ATG4B is mainly expressed in skeletal muscle, brain, heart, liver, and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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