Thermo Fisher Scientific ATG4B Polyclonal Antibody
ATG4B 단백질을 인식하는 Thermo Fisher Scientific의 다클론 항체로, Western blot 및 IHC에 적합합니다. 사람 유래 합성 펩타이드를 면역원으로 사용하였으며, 동결건조 형태로 제공됩니다. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:2,000 |
| Immunohistochemistry (IHC) | 1:200–1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from human ATG4B conjugated to an immunogenic carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not determined |
| Storage Conditions | Store at 4°C for short term. For long term, store at -20°C avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2274720 |
Product Specific Information
Reconstitute with 150 µL of distilled water.
Target Information
Cysteine protease ATG4B is an enzyme encoded by the ATG4B gene. Autophagy is a critical cellular homeostatic process that regulates the turnover of damaged organelles and proteins. ATG4B functions as a deubiquitin-like protease with dual roles in the core autophagy machinery. It cleaves the C-terminal amino acid of ATG8 family proteins (MAP1LC3, GABARAPL1, GABARAPL2, and GABARAP) to expose a C-terminal glycine. This exposed glycine is essential for ATG8 protein conjugation to phosphatidylethanolamine (PE) and membrane insertion, which are necessary for autophagy. ATG4B is mainly expressed in skeletal muscle, brain, heart, liver, and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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