Thermo Fisher Scientific Donkey anti-Mouse IgG (H+L) Secondary Antibody, AP
Thermo Fisher Scientific의 Donkey anti-Mouse IgG (H+L) Secondary Antibody, AP는 마우스 IgG 검출용 폴리클로날 항체로, Western blot, IHC, ELISA에 사용됩니다. 알칼리 포스파타제(AP) 결합형이며 높은 특이성과 감도를 제공합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,500 |
| ELISA | 1:2,000–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Full length native mouse IgG (purified) |
| Conjugate | AP |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | IgG fraction |
| Storage Buffer | 0.05M Tris HCl (pH 8.0), 0.15M NaCl, 10 mg/mL BSA, 0.001M MgCl₂, 0.001M ZnCl₂, 50% glycerol |
| Contains | 0.1% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10987188 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
PA1-28749 detects Mouse IgG from mouse samples.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting, or purification of their specified targets.
Secondary antibodies offer increased versatility, enabling use with various detection systems (e.g., HRP, AP, fluorescence). They provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
Additional Formats Available
- Alexa Fluor 488
- Alexa Fluor 594
- Biotin
- FITC
- HRP
- Qdot 525
- Qdot 605
- Qdot 625
- Texas Red
- TRITC
- Request custom conjugation
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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