Thermo Fisher Scientific BID Polyclonal Antibody
Thermo Fisher Scientific의 BID Polyclonal Antibody는 인간 BID 단백질을 인식하는 rabbit polyclonal 항체로, WB 및 IHC에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 액상 형태로 제공됩니다. 세포 사멸 연구용으로 권장됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 60 and 120 of human BH3 interacting domain death agonist (BID) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Phosphate/Tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
BID is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. It is a member of the BCL-2 family of cell death regulators and mediates mitochondrial damage induced by caspase-8 (CASP8). CASP8 cleaves BID, and the COOH-terminal part translocates to mitochondria, triggering cytochrome c release. BID acts as a pro-apoptotic factor linking caspase-8 activation to mitochondrial death machinery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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