Thermo Fisher Scientific CD54 (ICAM-1) Monoclonal Antibody (eBioKAT-1 (KAT-1, KAT1)), Super Bright 436, eBioscience
마우스 CD54(ICAM-1)을 인식하는 eBioKAT-1 단클론 항체로, Super Bright 436 형광체 결합. Flow Cytometry에 최적화되어 있으며 염증 반응 관련 연구에 유용. 405 nm 레이저로 여기, 436 nm에서 방출. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioKAT-1 (KAT-1, KAT1) |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2762737 |
Available Formats:
Product Specific Information
The eBioKAT-1 monoclonal antibody reacts with mouse CD54 (ICAM-1), a 95 kDa member of the immunoglobulin superfamily. CD54 is expressed at low levels on leukocytes and high endothelial venules, with expression increasing in response to inflammatory cytokines. ICAM-1 binds to LFA-1, facilitating transendothelial migration of T cells. ICAM-1-deficient mice show reduced leukocyte migration and impaired inflammatory responses.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes at ≤0.5 µg/test (100 µL final volume). Optimal titration recommended.
Fluorochrome Information:
Super Bright 436 is excited with the violet laser (405 nm) and emits at 436 nm. Recommended filter: 450/50 bandpass. Ensure instrument compatibility.
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
ICAM-1 (CD54) is an 85–110 kDa single-chain type I integral membrane glycoprotein with five immunoglobulin superfamily repeats, a transmembrane region, and a cytoplasmic domain. It binds to integrins CD11a/CD18 (LFA-1) and CD11b/CD18 (Mac-1), and serves as a receptor for Rhinovirus. ICAM-1 is expressed on activated endothelial cells, epithelial cells, fibroblasts, chondrocytes, B and T lymphocytes, monocytes, macrophages, dendritic cells, and neutrophils. Expression increases during inflammation and is also observed in carcinoma and melanoma cells. Soluble ICAM-1 is detectable in plasma and elevated in inflammatory conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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